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cd44-pe-cy7 im7 antibody  (Thermo Fisher)


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    Thermo Fisher cd44-pe-cy7 im7 antibody
    Cd44 Pe Cy7 Im7 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd44+im7+pe-cy7/anti+cd44/pmc09918539__41467_2023_36293_MOESM2_ESM-32-46-52
    Average 90 stars, based on 1 article reviews
    cd44-pe-cy7 im7 antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Article Snippet: Marker Clone Dye Supplier Reference RRID Dilution CD45 30-F11 BV421 BD 563890 AB_2651151 1/100 CD3 17A2 AF594 Biolegend 100240 AB_2563427 1/100 CD8 53-6.7 APC-H7 BD 560182 AB_1645237 1/100 CD4 GK1.5 PerCP-Cy5.5 Biolegend 100434 AB_893324 1/100 CD44 IM7 PE-Cy7 eBioscience 25-0441-82 AB_469623 1/100 CD62L Mel-14 AF647 Biolegend 104421 AB_493379 1/100 NK1.1 PK136 PE eBioscience 12-5941-83 AB_466050 1/100



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    Thymus implants repopulate T cells in Foxn1 nu athymic recipient mice. (a) Experimental scheme for fresh thymus implants. (b) Representative scatter plots of peripheral blood collected from Foxn1 nu mice implanted with thymus and labelled with anti‐CD3, CD45, CD4, CD8, <t>CD44</t> and CD62L antibodies. Cells were analysed using FACS to compare the T cell populations derived from recipient mice or donor tissue (blue = T cells from donor thymus in MHC‐Matched implant, pink = new T cells developed in the recipient after MHC‐matched thymus implants, grey = new T cells developed in the recipient after MHC‐Mismatched thymus implants). (c) Percentage of T cells/CD45+ cells in peripheral blood over time (weeks post post‐implant) in MHC‐mismatched thymus implantation compared to age‐matched wild‐type mice ( N = 3 or 4 mice/group from 2 experiments; 2‐way ANOVA P < 0.001; post‐hoc *** P < 0.001). (d) FACS analysis of peripheral blood T cells, 15 weeks post thymus implant (CM: central memory T cells; EM: effector memory T cells; N = 3 or 4 mice/group from 3 experiments; one‐way ANOVA; post‐hoc * P < 0.05, ** P < 0.01, *** P < 0.001). (e) Experimental scheme for cultured thymus implants using neonatal donor mice (aged 2–4 days). (f) Cellometer counts of cells in thymus culture media over time show peak at day 3 or 4 and decrease through day 8 ( N = 9–16 cultures per day in 1 experiment). (g) TEC populations in acutely isolated and cultured thymus were analysed by FACS ( N = 5 thymus per group from 1 experiment; P < 0.001, t ‐test). (h) Percentage of T cell subsets of total CD45 + cells in peripheral blood of Foxn1 nu athymic mice 15 weeks after cultured thymus implants ( N = 5 mice per group from two experiments).
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    Image Search Results


    Journal: eLife

    Article Title: The lncRNA Malat1 inhibits miR-15/16 to enhance cytotoxic T cell activation and memory cell formation

    doi: 10.7554/eLife.87900

    Figure Lengend Snippet:

    Article Snippet: Antibody , anti-CD44 PE-Cy7 (Clone-IM7, rat monoclonal) , eBiosciences , 25-0441-82 , (1:200).

    Techniques: Enzyme-linked Immunosorbent Assay, Sequencing, CRISPR

    Thymus implants repopulate T cells in Foxn1 nu athymic recipient mice. (a) Experimental scheme for fresh thymus implants. (b) Representative scatter plots of peripheral blood collected from Foxn1 nu mice implanted with thymus and labelled with anti‐CD3, CD45, CD4, CD8, CD44 and CD62L antibodies. Cells were analysed using FACS to compare the T cell populations derived from recipient mice or donor tissue (blue = T cells from donor thymus in MHC‐Matched implant, pink = new T cells developed in the recipient after MHC‐matched thymus implants, grey = new T cells developed in the recipient after MHC‐Mismatched thymus implants). (c) Percentage of T cells/CD45+ cells in peripheral blood over time (weeks post post‐implant) in MHC‐mismatched thymus implantation compared to age‐matched wild‐type mice ( N = 3 or 4 mice/group from 2 experiments; 2‐way ANOVA P < 0.001; post‐hoc *** P < 0.001). (d) FACS analysis of peripheral blood T cells, 15 weeks post thymus implant (CM: central memory T cells; EM: effector memory T cells; N = 3 or 4 mice/group from 3 experiments; one‐way ANOVA; post‐hoc * P < 0.05, ** P < 0.01, *** P < 0.001). (e) Experimental scheme for cultured thymus implants using neonatal donor mice (aged 2–4 days). (f) Cellometer counts of cells in thymus culture media over time show peak at day 3 or 4 and decrease through day 8 ( N = 9–16 cultures per day in 1 experiment). (g) TEC populations in acutely isolated and cultured thymus were analysed by FACS ( N = 5 thymus per group from 1 experiment; P < 0.001, t ‐test). (h) Percentage of T cell subsets of total CD45 + cells in peripheral blood of Foxn1 nu athymic mice 15 weeks after cultured thymus implants ( N = 5 mice per group from two experiments).

    Journal: Clinical & Translational Immunology

    Article Title: Improving thymus implantation for congenital athymia with interleukin‐7

    doi: 10.1002/cti2.1475

    Figure Lengend Snippet: Thymus implants repopulate T cells in Foxn1 nu athymic recipient mice. (a) Experimental scheme for fresh thymus implants. (b) Representative scatter plots of peripheral blood collected from Foxn1 nu mice implanted with thymus and labelled with anti‐CD3, CD45, CD4, CD8, CD44 and CD62L antibodies. Cells were analysed using FACS to compare the T cell populations derived from recipient mice or donor tissue (blue = T cells from donor thymus in MHC‐Matched implant, pink = new T cells developed in the recipient after MHC‐matched thymus implants, grey = new T cells developed in the recipient after MHC‐Mismatched thymus implants). (c) Percentage of T cells/CD45+ cells in peripheral blood over time (weeks post post‐implant) in MHC‐mismatched thymus implantation compared to age‐matched wild‐type mice ( N = 3 or 4 mice/group from 2 experiments; 2‐way ANOVA P < 0.001; post‐hoc *** P < 0.001). (d) FACS analysis of peripheral blood T cells, 15 weeks post thymus implant (CM: central memory T cells; EM: effector memory T cells; N = 3 or 4 mice/group from 3 experiments; one‐way ANOVA; post‐hoc * P < 0.05, ** P < 0.01, *** P < 0.001). (e) Experimental scheme for cultured thymus implants using neonatal donor mice (aged 2–4 days). (f) Cellometer counts of cells in thymus culture media over time show peak at day 3 or 4 and decrease through day 8 ( N = 9–16 cultures per day in 1 experiment). (g) TEC populations in acutely isolated and cultured thymus were analysed by FACS ( N = 5 thymus per group from 1 experiment; P < 0.001, t ‐test). (h) Percentage of T cell subsets of total CD45 + cells in peripheral blood of Foxn1 nu athymic mice 15 weeks after cultured thymus implants ( N = 5 mice per group from two experiments).

    Article Snippet: Antibodies for the T cell immunophenotyping panel were purchased from BD Biosciences: PerCP‐Cy5.5 CD45.1 (Clone: A20), PE‐Cy7 CD44 (Clone: IM7), APC CD3e (Clone: 145‐2C11), APC‐H7 CD4 (Clone: GK1.5), V450 CD45.2 (Clone: 104), V500 CD8 (Clone: 53–6.7) and BV605 CD62L (Clone: MEL‐14), AF488 CD45R/B220 (Clone: RA3‐6B2), PE CD45b (Clone: DX5), Alexa Fluor‐700 CD11c (Clone: N418), APC‐Cy7 CD11b (Clone: M1/70) and IL‐7R (Clone: SB/199).

    Techniques: Derivative Assay, Cell Culture, Isolation

    IL‐7 enhanced T cell development in peripheral blood by increasing the population of naïve T cells. (a) Experimental scheme treating Foxn1 nu athymic mice with cultured thymus transplants and IL‐7. Foxn1 nu athymic mice that received a cultured, MHC‐mismatched thymus (intramuscularly) were implanted with a mini‐osmotic pump containing PBS (Veh) or 5 μg of murine IL‐7 in PBS. (b, c) To compare the homeostatic proliferated T cell population, peripheral leukocytes were stained using IL‐7R antibody and the signal was analysed using FACS ( N = 4 mice/group from 1 experiment; two‐way repeated measures ANOVA; ** P < 0.01). (d) Peripheral blood collected from control or IL‐7‐treated mice transplanted with thymus was stained using anti‐CD45.1, CD45.2, CD3, CD4, CD8 CD44 and CD62L antibodies and analysed by FACS. T cell subpopulations were analysed and compared between the control and IL‐7‐treated groups based on CD44 and CD62L expression. (e–g) Data are presented as CD44 lo CD62L hi naïve populations per total cells (e), percentage of T cells per leukocytes (f) and absolute number of T cells in 100 μL of blood (g) on weeks 2, 4, 6, 8 and 12 after surgery ( N = 6 mice/group from 2 experiments; two‐way repeated measures ANOVA; * P < 0.05, ** P < 0.01 post‐hoc *** P < 0.001). (h) To compare TCR repertoire, the number of unique TCR clones was compared by RNA sequencing ( N = 3 mice/group from 1 experiment; two‐way repeated measures ANOVA; * P < 0.05).

    Journal: Clinical & Translational Immunology

    Article Title: Improving thymus implantation for congenital athymia with interleukin‐7

    doi: 10.1002/cti2.1475

    Figure Lengend Snippet: IL‐7 enhanced T cell development in peripheral blood by increasing the population of naïve T cells. (a) Experimental scheme treating Foxn1 nu athymic mice with cultured thymus transplants and IL‐7. Foxn1 nu athymic mice that received a cultured, MHC‐mismatched thymus (intramuscularly) were implanted with a mini‐osmotic pump containing PBS (Veh) or 5 μg of murine IL‐7 in PBS. (b, c) To compare the homeostatic proliferated T cell population, peripheral leukocytes were stained using IL‐7R antibody and the signal was analysed using FACS ( N = 4 mice/group from 1 experiment; two‐way repeated measures ANOVA; ** P < 0.01). (d) Peripheral blood collected from control or IL‐7‐treated mice transplanted with thymus was stained using anti‐CD45.1, CD45.2, CD3, CD4, CD8 CD44 and CD62L antibodies and analysed by FACS. T cell subpopulations were analysed and compared between the control and IL‐7‐treated groups based on CD44 and CD62L expression. (e–g) Data are presented as CD44 lo CD62L hi naïve populations per total cells (e), percentage of T cells per leukocytes (f) and absolute number of T cells in 100 μL of blood (g) on weeks 2, 4, 6, 8 and 12 after surgery ( N = 6 mice/group from 2 experiments; two‐way repeated measures ANOVA; * P < 0.05, ** P < 0.01 post‐hoc *** P < 0.001). (h) To compare TCR repertoire, the number of unique TCR clones was compared by RNA sequencing ( N = 3 mice/group from 1 experiment; two‐way repeated measures ANOVA; * P < 0.05).

    Article Snippet: Antibodies for the T cell immunophenotyping panel were purchased from BD Biosciences: PerCP‐Cy5.5 CD45.1 (Clone: A20), PE‐Cy7 CD44 (Clone: IM7), APC CD3e (Clone: 145‐2C11), APC‐H7 CD4 (Clone: GK1.5), V450 CD45.2 (Clone: 104), V500 CD8 (Clone: 53–6.7) and BV605 CD62L (Clone: MEL‐14), AF488 CD45R/B220 (Clone: RA3‐6B2), PE CD45b (Clone: DX5), Alexa Fluor‐700 CD11c (Clone: N418), APC‐Cy7 CD11b (Clone: M1/70) and IL‐7R (Clone: SB/199).

    Techniques: Cell Culture, Staining, Expressing, Clone Assay, RNA Sequencing Assay